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CRYOSURE

二甲基亞砜是一種重要的滲透型細(xì)胞保護(hù)劑。在深低溫（零下200度）保存細(xì)胞時(shí)凍存過程中，為防止細(xì)胞內(nèi)液冰晶形成、滲透壓改變、細(xì)胞結(jié)構(gòu)紊亂等導(dǎo)致的損傷，有必要使用含有DMSO冷凍保護(hù)劑。DMSO能夠快速穿透細(xì)胞膜進(jìn)入細(xì)胞中，降低冰點(diǎn)、延緩凍存過程，同時(shí)提高細(xì)胞內(nèi)離子濃度，減少細(xì)胞內(nèi)冰晶的形成，從而減少細(xì)胞損傷。深低溫時(shí)二甲基亞砜的細(xì)胞毒性受到抑制。復(fù)蘇時(shí)動(dòng)作要快，盡快洗掉二甲基亞砜，否則會(huì)造成對(duì)細(xì)胞嚴(yán)重的毒性。二甲基亞砜（DMSO）是目前的細(xì)胞凍存保護(hù)劑，但也是一種以細(xì)胞毒性很大的化學(xué)試驗(yàn)劑。研究結(jié)果表明，培養(yǎng)液中DMSO濃度為10%時(shí)，細(xì)胞生長(zhǎng)抑制率近100%；1‰濃度時(shí)抑制率為35%，即使是0.04‰的濃度，DMSO對(duì)細(xì)胞的生長(zhǎng)也有不利的影響。
1.	CryoSure-DMSO
	Dimethyl Sulfoxide Cryo-Preservative in Multidose Containers Fitted with Flipoff-Tearoff Closures. more info   Quantity Cat # 10 ml Multi-dose 10/case WAK-DMSO-10 50 ml Multi-dose 6/case WAK-DMSO-50 70 ml Multi-dose 6/case WAK-DMSO-70
2.	CryoSure-DEX40
	55% w/v Dimethyl Sulfoxide USP Grade, 5% w/v Dextran 40 USP Grade Cryo-Preservative in Multidose Containers Fitted with Flippoff-Tearoff Closure. more info   Quantity Cat # 8 ml Multi-dose 25/case WAK-DEX40-25

CryoSure-DMSO and CryoSure-DEX40 are registered as medical devices in the European Union according to the European Medical Device Directive 93/42/EEC.

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